MEDICINE (ENGLISH)
Bachelor	TR-NQF-HE: Level 6	QF-EHEA: First Cycle	EQF-LLL: Level 6

Ders Genel Tanıtım Bilgileri

Course Code:

1011002101

Ders İsmi:

Research Techniques in Biochemistry

Ders Yarıyılı:

Fall

Ders Kredileri:

Theoretical	Practical	Credit	ECTS
2	0	2	2

Language of instruction:

Ders Koşulu:

Ders İş Deneyimini Gerektiriyor mu?:

Type of course:

Department Elective

Course Level:

Bachelor

TR-NQF-HE:6. Master`s Degree

QF-EHEA:First Cycle

EQF-LLL:6. Master`s Degree

Mode of Delivery:

Face to face

Course Coordinator :

Assoc. Prof. Özge ÜNLÜ

Course Lecturer(s):

Öğr.Gör.Dr. Naile Fevziye MISIRLIOĞLU

Course Assistants:

Dersin Amaç ve İçeriği

Course Objectives:

The undergraduate basic biochemistry teaching techniques and laboratory has
become an essential feature in the training of students in biochemistry, chemistry and molecular biology and related biological sciences. Students must acquire extensive experience working with biomolecules in the laboratory and a formal laboratory course is usually the first step to gain that experience. This course prepares students for participation in future research and development projects

Course Content:

Reaktif olarak su, laboratuar güvenliği ve biyogüvenlik, Laboratuar defteri ve rapor hazırlama, Biyolojik örneklerin hazırlanması, homojenizasyon /parçalama, santrifüj, membran filtrasyon teknikleri, çöktürme teknikleri, kromatografik yöntemler, elektroforetik yöntemler; protein (SDS-PAGE, IEF, 2D-PAGE) ve DNA elektroforezi, İmmünolojik teknikler

Learning Outcomes

The students who have succeeded in this course;

Learning Outcomes

1 - Knowledge
Theoretical - Conceptual
1) Be able to use the basic theoretical and practical issues involved in the separation and analysis of biological molecules
2) Gain an ability to think critically and to analyze biochemical problems
3) Gain an ability to work with people in teams to solve biochemical problems and enhance independent work capability
4) Be able to interpret data, carry out experiments and evaluate results
2 - Skills
Cognitive - Practical
1) Gain an ability to work effectively in laboratory environment and to use biochemical instrumentation and procedures
2) Gain an understanding on preparation of laboratory notebook and report preparation, data analysis and the use of computers in experiments
3 - Competences
Communication and Social Competence
Learning Competence
Field Specific Competence
Competence to Work Independently and Take Responsibility

Ders Akış Planı

Week	Subject	Related Preparation
1)	Water as a reagent, Water Purification and types of water for experiments in Biochemistry. Laboratory safety and Biosafety Rules, Laboratory notebook and Preparation of reports, Errors, Graphing	none
2)	Biological sample preparation opportunities and limitings. Choice of starting material, Cell disruption/homogenization: Choice of method, Efficiency of disruptive method.	none
3)	Mechanical Methods; Homogenizators, Blenders, grinders, agitation with abbresives, liquid and solid extrusion, ultrasonication Chemical Methods ; heat treatment, freeze-thaw, desiccation, osmotic shock, lytic enzymes, alkali treatment, detergents, etc.	none
4)	Centrifugation; Theory, Classification, Types of Centrifuges, Differential centrifugation, Density gradient centrifugation, Membrane filtration techniques	none
5)	Concentration of the extract; Ultrafiltration, Lyophilization, Precipitation techniques( salting-out, salting-in, isoelectric, organic solvents and polymers	none
6)	Chromatography, Theory, applications, types of Chromatography, Paper and Thin Layer Chromatography, Basic principles and theory of liquid chromatography,	none

Sources

Course Notes / Textbooks:

Keith Wilson and John Walker, Principles and Techniques of Biochemistry and Molecular Biology (2010)
R. Boyer, Modern Experimental Biochemistry, Benjamin Cummings, (2000)
R. Switzer, L. Garrity, Experimental Biochemistry, W. H. Freeman Company, (1999)
D.J. Holme, H. Peck, Analytical Biochemistry, Prentice Hall, (1998)
Gallagher, S.R. and Wiley E.A. Current Protocols Essential Laboratory Techniques, Wiley Press, (2008)

References:

Ders - Program Öğrenme Kazanım İlişkisi

Ders Öğrenme Kazanımları	1	3	4	5	2	6
Program Outcomes
1) Explain the basic structure, development and normal functioning of the human body at the level of molecules, cells, tissues, organs and systems.
2) Questions the abnormal structure and functioning of the human body, explains it with information based on qualified scientific research, evaluates the causes of diseases by considering the interaction with the individual and his environment.
3) Evaluates clinical decision making and management processes of diseases under the guidance of evidence-based medicine practices.
4) Defines the concepts of health and disease in individual and social contexts, explains health seeking and health protection behaviors, national health service delivery and administrative processes.
5) Knows the research processes that form the basis of medical knowledge, has a level of foreign language knowledge to follow the developments in this field.
6) Takes medical history from the applicant/patient and their relatives.
7) Performs physical examination of individuals, evaluates diagnostic tests, manages diagnosis and treatment processes by using appropriate process steps.
8) Applies medical interventions to individuals for diagnosis, treatment or prevention.
9) Plans and realizes practices for the protection and improvement of health in individual and social dimensions.
10) Plans a scientific research, implements and evaluates the results.
11) Demonstrates lifelong learning behavior by making use of scientific and technological developments for professional and social changes.
12) Fulfills its responsibilities as a physician within the framework of professional values, ethical principles and legal regulations, regardless of language, religion, race, gender, social and cultural discrimination in the individual and society it serves.
13) He / she works in teams with his colleagues and other healthcare professionals in the processes of protecting and improving health in individual and social dimensions, and managing diseases.
14) Organizes and keeps records of health and disease related data obtained from individuals and society in medical and administrative contexts.
15) It strives to protect and develop the health of the individual and the society and to provide health services for the benefit of the individuals who make up the society.

Ders - Öğrenme Kazanımı İlişkisi

No Effect	1 Lowest	2 Low	3 Average	4 High	5 Highest

	Program Outcomes	Level of Contribution
1)	Explain the basic structure, development and normal functioning of the human body at the level of molecules, cells, tissues, organs and systems.
2)	Questions the abnormal structure and functioning of the human body, explains it with information based on qualified scientific research, evaluates the causes of diseases by considering the interaction with the individual and his environment.
3)	Evaluates clinical decision making and management processes of diseases under the guidance of evidence-based medicine practices.
4)	Defines the concepts of health and disease in individual and social contexts, explains health seeking and health protection behaviors, national health service delivery and administrative processes.
5)	Knows the research processes that form the basis of medical knowledge, has a level of foreign language knowledge to follow the developments in this field.
6)	Takes medical history from the applicant/patient and their relatives.
7)	Performs physical examination of individuals, evaluates diagnostic tests, manages diagnosis and treatment processes by using appropriate process steps.
8)	Applies medical interventions to individuals for diagnosis, treatment or prevention.
9)	Plans and realizes practices for the protection and improvement of health in individual and social dimensions.
10)	Plans a scientific research, implements and evaluates the results.
11)	Demonstrates lifelong learning behavior by making use of scientific and technological developments for professional and social changes.
12)	Fulfills its responsibilities as a physician within the framework of professional values, ethical principles and legal regulations, regardless of language, religion, race, gender, social and cultural discrimination in the individual and society it serves.
13)	He / she works in teams with his colleagues and other healthcare professionals in the processes of protecting and improving health in individual and social dimensions, and managing diseases.
14)	Organizes and keeps records of health and disease related data obtained from individuals and society in medical and administrative contexts.
15)	It strives to protect and develop the health of the individual and the society and to provide health services for the benefit of the individuals who make up the society.

Öğrenme Etkinliği ve Öğretme Yöntemleri

Anlatım
Bireysel çalışma ve ödevi
Soru cevap/ Tartışma

Ölçme ve Değerlendirme Yöntemleri ve Kriterleri

Yazılı Sınav (Açık uçlu sorular, çoktan seçmeli, doğru yanlış, eşleştirme, boşluk doldurma, sıralama)

Assessment & Grading

Semester Requirements	Number of Activities	Level of Contribution
Midterms	1	% 40
Semester Final Exam	1	% 60
total		% 100
PERCENTAGE OF SEMESTER WORK		% 40
PERCENTAGE OF FINAL WORK		% 60
total		% 100

İş Yükü ve AKTS Kredisi Hesaplaması

Activities	Number of Activities	Duration (Hours)	Workload
Course Hours	14	2	28
Total Workload			28