MEDICAL LABORATORY TECHNIQUES
Associate	TR-NQF-HE: Level 5	QF-EHEA: Short Cycle	EQF-LLL: Level 5

Ders Genel Tanıtım Bilgileri

Course Code:

2020002003

Ders İsmi:

Genetical Analysis

Ders Yarıyılı:

Spring

Ders Kredileri:

Theoretical	Practical	Credit	ECTS
2	0	2	3

Language of instruction:

Ders Koşulu:

Ders İş Deneyimini Gerektiriyor mu?:

Type of course:

Bölüm Seçmeli

Course Level:

Associate

TR-NQF-HE:5. Master`s Degree

QF-EHEA:Short Cycle

EQF-LLL:5. Master`s Degree

Mode of Delivery:

Face to face

Course Coordinator :

Dr.Öğr.Üyesi Hatice Nur HALİPÇİ TOPSAKAL

Course Lecturer(s):

Öğr.Gör. Senem ASLAN ÖZTÜRK

Course Assistants:

Dersin Amaç ve İçeriği

Course Objectives:

In this course, it is aimed that the students have information about the studies in the laboratories where genetic analysis is carried out.

Course Content:

Laboratory Safety, Structure of DNA, Source of Genetic Diversity
DNA Isolation, Agaro gel electrophoresis, Restriction Enzyme Analysis, Polymerase Chain Reaction, DNA sequence analysis, Recombinant DNA technology, Recombinant DNA technology, Cytogenetic tests, Peripheral blood culture preparation, Karyotype analysis, Fluorescent in situ hybridization (FISH) technique, Prenatal genetic tests Human chromosomal disorders are the course content.

Learning Outcomes

The students who have succeeded in this course;

Learning Outcomes

1 - Knowledge
Theoretical - Conceptual
1) Laboratory Safety knows the structure of DNA, the Source of Genetic Diversity.
2) Understands how DNA isolation is.
3) Knows Agaro gel electrophoresis.
4) Knows Restriction Enzyme Analysis.
5) Knows and tests the Polymerase Chain Reaction
6) Knows DNA sequence analysis.
7) Have general information about recombinant DNA technology.
8) Lists cytogenetic tests.
9) Prepare a peripheral blood culture
10) Knows karyotype analysis.
11) Knows fluorescent in situ hybridization (FISH) technique.
12) Prenatal genetic testing enumerates Human chromosomal aberrations.
2 - Skills
Cognitive - Practical
3 - Competences
Communication and Social Competence
Learning Competence
Field Specific Competence
Competence to Work Independently and Take Responsibility

Ders Akış Planı

Week	Subject	Related Preparation
1)	Laboratory Safety, Structure of DNA, Source of Genetic Diversity	Temel ve İleri Moleküler Biyoloji Yöntemleri, Prof.Güler Temizkan Genetik ve Proteomik Analizlar, Prof.Dr.Nazlı Arda Öğretim görevlisinin ders notları
2)	DNA Isolation	Temel ve İleri Moleküler Biyoloji Yöntemleri, Prof.Güler Temizkan Genetik ve Proteomik Analizlar, Prof.Dr.Nazlı Arda Öğretim görevlisinin ders notları
3)	Agaro gel electrophoresis	Temel ve İleri Moleküler Biyoloji Yöntemleri, Prof.Güler Temizkan Genetik ve Proteomik Analizlar, Prof.Dr.Nazlı Arda Öğretim görevlisinin ders notları
4)	Restriction Enzyme Analysis	Temel ve İleri Moleküler Biyoloji Yöntemleri, Prof.Güler Temizkan Genetik ve Proteomik Analizlar, Prof.Dr.Nazlı Arda Öğretim görevlisinin ders notları
5)	Polymerase Chain Reaction	Temel ve İleri Moleküler Biyoloji Yöntemleri, Prof.Güler Temizkan Genetik ve Proteomik Analizlar, Prof.Dr.Nazlı Arda Öğretim görevlisinin ders notları
6)	DNA sequence analysis	Temel ve İleri Moleküler Biyoloji Yöntemleri, Prof.Güler Temizkan Genetik ve Proteomik Analizlar, Prof.Dr.Nazlı Arda Öğretim görevlisinin ders notları
7)	Recombinant DNA technology-2	Temel ve İleri Moleküler Biyoloji Yöntemleri, Prof.Güler Temizkan Genetik ve Proteomik Analizlar, Prof.Dr.Nazlı Arda Öğretim görevlisinin ders notları
8)	Midterm
9)	Recombinant DNA technology-2	Temel ve İleri Moleküler Biyoloji Yöntemleri, Prof.Güler Temizkan Genetik ve Proteomik Analizlar, Prof.Dr.Nazlı Arda Öğretim görevlisinin ders notları
10)	Cytogenetic studies	Temel ve İleri Moleküler Biyoloji Yöntemleri, Prof.Güler Temizkan Genetik ve Proteomik Analizlar, Prof.Dr.Nazlı Arda Öğretim görevlisinin ders notları
11)	Peripheral blood culture preparation	Temel ve İleri Moleküler Biyoloji Yöntemleri, Prof.Güler Temizkan Genetik ve Proteomik Analizlar, Prof.Dr.Nazlı Arda Öğretim görevlisinin ders notları
12)	Karyotype analysis	Temel ve İleri Moleküler Biyoloji Yöntemleri, Prof.Güler Temizkan Genetik ve Proteomik Analizlar, Prof.Dr.Nazlı Arda Öğretim görevlisinin ders notları
13)	Fluorescent in situ hybridization (FISH) technique	Temel ve İleri Moleküler Biyoloji Yöntemleri, Prof.Güler Temizkan Genetik ve Proteomik Analizlar, Prof.Dr.Nazlı Arda Öğretim görevlisinin ders notları
14)	Prenatal genetic testing, Human chromosome disorders-1	Temel ve İleri Moleküler Biyoloji Yöntemleri, Prof.Güler Temizkan Genetik ve Proteomik Analizlar, Prof.Dr.Nazlı Arda Öğretim görevlisinin ders notları
15)	Prenatal genetic testing, Human chromosome disorders-2	Temel ve İleri Moleküler Biyoloji Yöntemleri, Prof.Güler Temizkan Genetik ve Proteomik Analizlar, Prof.Dr.Nazlı Arda Öğretim görevlisinin ders notları
16)	Final Exam

Sources

Course Notes / Textbooks:	Temel ve İleri Moleküler Biyoloji Yöntemleri, Prof.Güler Temizkan Genetik ve Proteomik Analizlar, Prof.Dr.Nazlı Arda Öğretim görevlisinin ders notları
References:	Temel ve İleri Moleküler Biyoloji Yöntemleri, Prof.Güler Temizkan Genetik ve Proteomik Analizlar, Prof.Dr.Nazlı Arda Öğretim görevlisinin ders notları

Ders - Program Öğrenme Kazanım İlişkisi

Ders Öğrenme Kazanımları	1	2	3	4	5	6	7	8	9	10	11	12
Program Outcomes
1) Have basic knowledge about normal structure-functions of human systems.
2) Gains knowledge about the workflow and organization in medical laboratories.
3) Have basic theoretical and applied knowledge about the field.
4) Knows manual and automatic analysis methods in the Medical Laboratory and produces solutions in case of malfunctions.
5) Takes the samples that the specialist physician deems necessary from the patient in an appropriate way, and performs the sample transfer under appropriate conditions.
6) Performs routine examinations in the medical laboratory, follows the sample, concludes the analysis and establishes the relationship with the diseases.
7) Accurately obtains the findings, organizes them, presents them for sharing and use in accordance with the purpose.
8) Takes safety precautions in the medical laboratory, applies personal safety rules and creates a safe laboratory environment.
9) In the working environment; applies first aid and emergency care in accordance with the guide/directive in emergency situations.
10) Takes responsibility in a given task and carries it out individually and takes responsibility in working groups when necessary.
11) Gains proficiency in recognizing devices related to the field and fixing simple problems.
12) It carries out the necessary activities for the professional development of the employees under its responsibility.
13) Participates in the necessary programs, vocational in-service training activities and other studies in order to improve herself personally and professionally by updating the knowledge, skills and competencies related to her field with the awareness of lifelong learning.
14) It follows the developments in technology and science and has the consciousness of constantly renewing itself.
15) Gains the ability to communicate effectively with physicians ,colleagues, other healthcare professionals and patients in an ethical framework., other healthcare professionals and patients in an ethical framework.
16) Uses his/her mother tongue at a good level in oral and written communication for his/her profession.
17) He/she makes preliminary preparations for manual and automatic studies before analysis.
18) Applies basic tests related to different areas of the medical laboratory.
19) Uses medical laboratory equipment in accordance with its rules and techniques, ensures that they work in the most efficient way by controlling and maintaining them.
20) Have sufficient awareness of environmental protection and occupational safety.

Ders - Öğrenme Kazanımı İlişkisi

No Effect	1 Lowest	2 Low	3 Average	4 High	5 Highest

	Program Outcomes	Level of Contribution
1)	Have basic knowledge about normal structure-functions of human systems.
2)	Gains knowledge about the workflow and organization in medical laboratories.
3)	Have basic theoretical and applied knowledge about the field.
4)	Knows manual and automatic analysis methods in the Medical Laboratory and produces solutions in case of malfunctions.
5)	Takes the samples that the specialist physician deems necessary from the patient in an appropriate way, and performs the sample transfer under appropriate conditions.
6)	Performs routine examinations in the medical laboratory, follows the sample, concludes the analysis and establishes the relationship with the diseases.
7)	Accurately obtains the findings, organizes them, presents them for sharing and use in accordance with the purpose.
8)	Takes safety precautions in the medical laboratory, applies personal safety rules and creates a safe laboratory environment.
9)	In the working environment; applies first aid and emergency care in accordance with the guide/directive in emergency situations.
10)	Takes responsibility in a given task and carries it out individually and takes responsibility in working groups when necessary.
11)	Gains proficiency in recognizing devices related to the field and fixing simple problems.
12)	It carries out the necessary activities for the professional development of the employees under its responsibility.
13)	Participates in the necessary programs, vocational in-service training activities and other studies in order to improve herself personally and professionally by updating the knowledge, skills and competencies related to her field with the awareness of lifelong learning.
14)	It follows the developments in technology and science and has the consciousness of constantly renewing itself.
15)	Gains the ability to communicate effectively with physicians ,colleagues, other healthcare professionals and patients in an ethical framework., other healthcare professionals and patients in an ethical framework.
16)	Uses his/her mother tongue at a good level in oral and written communication for his/her profession.
17)	He/she makes preliminary preparations for manual and automatic studies before analysis.
18)	Applies basic tests related to different areas of the medical laboratory.
19)	Uses medical laboratory equipment in accordance with its rules and techniques, ensures that they work in the most efficient way by controlling and maintaining them.
20)	Have sufficient awareness of environmental protection and occupational safety.

Öğrenme Etkinliği ve Öğretme Yöntemleri

Anlatım
Beyin fırtınası /Altı şapka
Course

Ölçme ve Değerlendirme Yöntemleri ve Kriterleri

Yazılı Sınav (Açık uçlu sorular, çoktan seçmeli, doğru yanlış, eşleştirme, boşluk doldurma, sıralama)

Assessment & Grading

Semester Requirements	Number of Activities	Level of Contribution
Midterms	1	% 40
Semester Final Exam	1	% 60
total		% 100
PERCENTAGE OF SEMESTER WORK		% 40
PERCENTAGE OF FINAL WORK		% 60
total		% 100

İş Yükü ve AKTS Kredisi Hesaplaması

Activities	Number of Activities	Duration (Hours)	Workload
Course Hours	14	2	28
Midterms	1	25	25
Final	1	25	25
Total Workload			78