BİLGİ PAKETİ / DERS KATALOĞU
2020002002 Cell Culture Methods
Degree Programs
MEDICAL LABORATORY TECHNIQUES
Diploma SupplementNational Qualifications
MEDICAL LABORATORY TECHNIQUES
Associate TR-NQF-HE: Level 5 QF-EHEA: Short Cycle EQF-LLL: Level 5

Ders Genel Tanıtım Bilgileri

Course Code: 2020002002
Ders İsmi: Cell Culture Methods
Ders Yarıyılı: Fall
Ders Kredileri:
Theoretical Practical Credit ECTS
2 0 2 3
Language of instruction: TR
Ders Koşulu:
Ders İş Deneyimini Gerektiriyor mu?: No
Type of course: Bölüm Seçmeli
Course Level:
Associate TR-NQF-HE:5. Master`s Degree QF-EHEA:Short Cycle EQF-LLL:5. Master`s Degree
Mode of Delivery: Face to face
Course Coordinator : Dr.Öğr.Üyesi Hatice Nur HALİPÇİ TOPSAKAL
Course Lecturer(s):
Course Assistants:

Dersin Amaç ve İçeriği

Course Objectives: The aim of this course is to provide students with basic and detailed information about basic cell culture laboratory layout, cell culture, cell culture techniques, cell culture usage areas and stem cell concepts.
Course Content: This course; Introduction to cell culture, basic cell culture laboratory layout, materials used in cell culture and their preparation, methods used in cell selection. Cell culture techniques, Primary culture and passage, Cell culture usage areas, Sterilization and contamination in cell culture, safety measures in cell culture, freezing cells, working with cells that have been frozen, physical and chemical factors affecting the development of cells in culture, Stem cells and their application areas, cancer stem cell, viability test of cells.

Learning Outcomes

The students who have succeeded in this course;
Learning Outcomes
1 - Knowledge
Theoretical - Conceptual
1) Defines cell culture and knows for what purpose it is used
2) Learns cell culture techniques and usage areas.
3) Knows the cell culture laboratory layout, materials and equipment used.
4) Gains information about sterilization and contamination in cell culture.
5) Knows and applies safety precautions in cell culture.
6) Gains basic information about freezing cells and working with cryopreserved cells.
7) Knows the physical and chemical factors affecting the development of cells in culture.
8) Midterm exam
9) Gains basic information about stem cells and cancer stem cells.
10) Gains basic information about cell viability tests.
2 - Skills
Cognitive - Practical
3 - Competences
Communication and Social Competence
Learning Competence
Field Specific Competence
Competence to Work Independently and Take Responsibility

Ders Akış Planı

Week Subject Related Preparation
1) Introduction to cell culture and its history Lecture notes of the lecturer giving the course KÖK HÜCRE. Biyolojisi, Türleri ve Tedavide Kullanımları, ALP CAN, 2013 Doku ve Hücre Kültürü Teknikleri, İsmail Kocaçalışkan, 2017 Basic Cell Culture Protocols, Cheryl D. HelgasonCindy L. Miller, 2005
2) What is cell culture, for what purpose is it used? Dersi veren öğretim görevlisinin ders notları KÖK HÜCRE. Biyolojisi, Türleri ve Tedavide Kullanımları, ALP CAN, 2013 Doku ve Hücre Kültürü Teknikleri, İsmail Kocaçalışkan, 2017 Basic Cell Culture Protocols, Cheryl D. HelgasonCindy L. Miller, 2005
3) Cell culture techniques and uses Dersi veren öğretim görevlisinin ders notları KÖK HÜCRE. Biyolojisi, Türleri ve Tedavide Kullanımları, ALP CAN, 2013 Doku ve Hücre Kültürü Teknikleri, İsmail Kocaçalışkan, 2017 Basic Cell Culture Protocols, Cheryl D. HelgasonCindy L. Miller, 2005
4) Materials, equipment and laboratory layout used in cell culture. Dersi veren öğretim görevlisinin ders notları KÖK HÜCRE. Biyolojisi, Türleri ve Tedavide Kullanımları, ALP CAN, 2013 Doku ve Hücre Kültürü Teknikleri, İsmail Kocaçalışkan, 2017 Basic Cell Culture Protocols, Cheryl D. HelgasonCindy L. Miller, 2005
5) Cell selection methods Dersi veren öğretim görevlisinin ders notları KÖK HÜCRE. Biyolojisi, Türleri ve Tedavide Kullanımları, ALP CAN, 2013 Doku ve Hücre Kültürü Teknikleri, İsmail Kocaçalışkan, 2017 Basic Cell Culture Protocols, Cheryl D. HelgasonCindy L. Miller, 2005
6) Aseptic techniques and sterilization in cell culture Dersi veren öğretim görevlisinin ders notları KÖK HÜCRE. Biyolojisi, Türleri ve Tedavide Kullanımları, ALP CAN, 2013 Doku ve Hücre Kültürü Teknikleri, İsmail Kocaçalışkan, 2017 Basic Cell Culture Protocols, Cheryl D. HelgasonCindy L. Miller, 2005
7) Cell culture and contamination Dersi veren öğretim görevlisinin ders notları KÖK HÜCRE. Biyolojisi, Türleri ve Tedavide Kullanımları, ALP CAN, 2013 Doku ve Hücre Kültürü Teknikleri, İsmail Kocaçalışkan, 2017 Basic Cell Culture Protocols, Cheryl D. HelgasonCindy L. Miller, 2005
8) Midterm exam
9) Safety precautions in cell culture Dersi veren öğretim görevlisinin ders notları KÖK HÜCRE. Biyolojisi, Türleri ve Tedavide Kullanımları, ALP CAN, 2013 Doku ve Hücre Kültürü Teknikleri, İsmail Kocaçalışkan, 2017 Basic Cell Culture Protocols, Cheryl D. HelgasonCindy L. Miller, 2005
10) Freezing of cells Dersi veren öğretim görevlisinin ders notları KÖK HÜCRE. Biyolojisi, Türleri ve Tedavide Kullanımları, ALP CAN, 2013 Doku ve Hücre Kültürü Teknikleri, İsmail Kocaçalışkan, 2017 Basic Cell Culture Protocols, Cheryl D. HelgasonCindy L. Miller, 2005
11) Working with cryopreserved cells. Dersi veren öğretim görevlisinin ders notları KÖK HÜCRE. Biyolojisi, Türleri ve Tedavide Kullanımları, ALP CAN, 2013 Doku ve Hücre Kültürü Teknikleri, İsmail Kocaçalışkan, 2017 Basic Cell Culture Protocols, Cheryl D. HelgasonCindy L. Miller, 2005
12) Physical and chemical factors affecting the growth of cells in culture. Dersi veren öğretim görevlisinin ders notları KÖK HÜCRE. Biyolojisi, Türleri ve Tedavide Kullanımları, ALP CAN, 2013 Doku ve Hücre Kültürü Teknikleri, İsmail Kocaçalışkan, 2017 Basic Cell Culture Protocols, Cheryl D. HelgasonCindy L. Miller, 2005
13) Stem cells and application areas Dersi veren öğretim görevlisinin ders notları KÖK HÜCRE. Biyolojisi, Türleri ve Tedavide Kullanımları, ALP CAN, 2013 Doku ve Hücre Kültürü Teknikleri, İsmail Kocaçalışkan, 2017 Basic Cell Culture Protocols, Cheryl D. HelgasonCindy L. Miller, 2005
14) Cancer stem cell Dersi veren öğretim görevlisinin ders notları KÖK HÜCRE. Biyolojisi, Türleri ve Tedavide Kullanımları, ALP CAN, 2013 Doku ve Hücre Kültürü Teknikleri, İsmail Kocaçalışkan, 2017 Basic Cell Culture Protocols, Cheryl D. HelgasonCindy L. Miller, 2005
15) Cell viability tests Dersi veren öğretim görevlisinin ders notları KÖK HÜCRE. Biyolojisi, Türleri ve Tedavide Kullanımları, ALP CAN, 2013 Doku ve Hücre Kültürü Teknikleri, İsmail Kocaçalışkan, 2017 Basic Cell Culture Protocols, Cheryl D. HelgasonCindy L. Miller, 2005
16) Final exam

Sources

Course Notes / Textbooks: Dersi veren öğretim görevlisinin ders notları
KÖK HÜCRE. Biyolojisi, Türleri ve Tedavide Kullanımları, ALP CAN, 2013
Doku ve Hücre Kültürü Teknikleri, İsmail Kocaçalışkan, 2017
Basic Cell Culture Protocols, Cheryl D. HelgasonCindy L. Miller, 2005
References: Lecture notes of the lecturer giving the course
KÖK HÜCRE. Biyolojisi, Türleri ve Tedavide Kullanımları, ALP CAN, 2013
Doku ve Hücre Kültürü Teknikleri, İsmail Kocaçalışkan, 2017
Basic Cell Culture Protocols, Cheryl D. HelgasonCindy L. Miller, 2005

Ders - Program Öğrenme Kazanım İlişkisi

Ders Öğrenme Kazanımları

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2

3

4

5

6

7

8

9

10
Program Outcomes
1) Have basic knowledge about normal structure-functions of human systems.
2) Gains knowledge about the workflow and organization in medical laboratories.
3) Have basic theoretical and applied knowledge about the field.
4) Knows manual and automatic analysis methods in the Medical Laboratory and produces solutions in case of malfunctions.
5) Takes the samples that the specialist physician deems necessary from the patient in an appropriate way, and performs the sample transfer under appropriate conditions.
6) Performs routine examinations in the medical laboratory, follows the sample, concludes the analysis and establishes the relationship with the diseases.
7) Accurately obtains the findings, organizes them, presents them for sharing and use in accordance with the purpose.
8) Takes safety precautions in the medical laboratory, applies personal safety rules and creates a safe laboratory environment.
9) In the working environment; applies first aid and emergency care in accordance with the guide/directive in emergency situations.
10) Takes responsibility in a given task and carries it out individually and takes responsibility in working groups when necessary.
11) Gains proficiency in recognizing devices related to the field and fixing simple problems.
12) It carries out the necessary activities for the professional development of the employees under its responsibility.
13) Participates in the necessary programs, vocational in-service training activities and other studies in order to improve herself personally and professionally by updating the knowledge, skills and competencies related to her field with the awareness of lifelong learning.
14) It follows the developments in technology and science and has the consciousness of constantly renewing itself.
15) Gains the ability to communicate effectively with physicians ,colleagues, other healthcare professionals and patients in an ethical framework., other healthcare professionals and patients in an ethical framework.
16) Uses his/her mother tongue at a good level in oral and written communication for his/her profession.
17) He/she makes preliminary preparations for manual and automatic studies before analysis.
18) Applies basic tests related to different areas of the medical laboratory.
19) Uses medical laboratory equipment in accordance with its rules and techniques, ensures that they work in the most efficient way by controlling and maintaining them.
20) Have sufficient awareness of environmental protection and occupational safety.

Ders - Öğrenme Kazanımı İlişkisi

No Effect 1 Lowest 2 Low 3 Average 4 High 5 Highest
           
Program Outcomes Level of Contribution
1) Have basic knowledge about normal structure-functions of human systems.
2) Gains knowledge about the workflow and organization in medical laboratories.
3) Have basic theoretical and applied knowledge about the field.
4) Knows manual and automatic analysis methods in the Medical Laboratory and produces solutions in case of malfunctions.
5) Takes the samples that the specialist physician deems necessary from the patient in an appropriate way, and performs the sample transfer under appropriate conditions.
6) Performs routine examinations in the medical laboratory, follows the sample, concludes the analysis and establishes the relationship with the diseases.
7) Accurately obtains the findings, organizes them, presents them for sharing and use in accordance with the purpose.
8) Takes safety precautions in the medical laboratory, applies personal safety rules and creates a safe laboratory environment.
9) In the working environment; applies first aid and emergency care in accordance with the guide/directive in emergency situations.
10) Takes responsibility in a given task and carries it out individually and takes responsibility in working groups when necessary.
11) Gains proficiency in recognizing devices related to the field and fixing simple problems.
12) It carries out the necessary activities for the professional development of the employees under its responsibility.
13) Participates in the necessary programs, vocational in-service training activities and other studies in order to improve herself personally and professionally by updating the knowledge, skills and competencies related to her field with the awareness of lifelong learning.
14) It follows the developments in technology and science and has the consciousness of constantly renewing itself.
15) Gains the ability to communicate effectively with physicians ,colleagues, other healthcare professionals and patients in an ethical framework., other healthcare professionals and patients in an ethical framework.
16) Uses his/her mother tongue at a good level in oral and written communication for his/her profession.
17) He/she makes preliminary preparations for manual and automatic studies before analysis.
18) Applies basic tests related to different areas of the medical laboratory.
19) Uses medical laboratory equipment in accordance with its rules and techniques, ensures that they work in the most efficient way by controlling and maintaining them.
20) Have sufficient awareness of environmental protection and occupational safety.

Öğrenme Etkinliği ve Öğretme Yöntemleri

Anlatım
Course
Homework
Soru cevap/ Tartışma

Ölçme ve Değerlendirme Yöntemleri ve Kriterleri

Yazılı Sınav (Açık uçlu sorular, çoktan seçmeli, doğru yanlış, eşleştirme, boşluk doldurma, sıralama)

Assessment & Grading

Semester Requirements Number of Activities Level of Contribution
Midterms 1 % 40
Semester Final Exam 1 % 60
total % 100
PERCENTAGE OF SEMESTER WORK % 40
PERCENTAGE OF FINAL WORK % 60
total % 100

İş Yükü ve AKTS Kredisi Hesaplaması

Activities Number of Activities Duration (Hours) Workload
Course Hours 14 2 28
Study Hours Out of Class 14 2 28
Midterms 1 22 22
Final 1 25 25
Total Workload 103