DISINFECTION, STERILIZATION AND ANTISEPSY TECHNICIAN
Associate	TR-NQF-HE: Level 5	QF-EHEA: Short Cycle	EQF-LLL: Level 5

Ders Genel Tanıtım Bilgileri

Course Code:

2015002004

Ders İsmi:

Sterilization Principles in Cell Culture

Ders Yarıyılı:

Fall
Spring

Ders Kredileri:

Theoretical	Practical	Credit	ECTS
2	0	2	3

Language of instruction:

Ders Koşulu:

Ders İş Deneyimini Gerektiriyor mu?:

Type of course:

Bölüm Seçmeli

Course Level:

Associate

TR-NQF-HE:5. Master`s Degree

QF-EHEA:Short Cycle

EQF-LLL:5. Master`s Degree

Mode of Delivery:

E-Learning

Course Coordinator :

Dr.Öğr.Üyesi Hatice Nur HALİPÇİ TOPSAKAL

Course Lecturer(s):

Dr.Öğr.Üyesi Burcu BİLTEKİN

Course Assistants:

Dersin Amaç ve İçeriği

Course Objectives:	The aim of this course is to learn the principles of cell culture, to have an idea about all the materials to be used in the work environment, to see what contamination causes in cell culture, to perform decontamination, to provide basic learning of each cell culture step where sterilization and disinfection are important.
Course Content:	The basic principles of cell culture, practical aspects, decontamination problem, which is frequently encountered in practice, sensitivity to aseptic working, why cell culture is important are the content of this course. How disinfection and sterilization applications are performed in infertility laboratories, which is one of the study areas, indicates the content of this course.

Learning Outcomes

The students who have succeeded in this course;

Learning Outcomes

1 - Knowledge
Theoretical - Conceptual
1) Learn the terms cell-tissue-culture.
2) Knows cell passage methods.
3) Understands why cell culture laboratory is important in many experimental applications.
4) Aware of the importance of working in aseptic conditions with intermediate processes of cultural steps.
5) Knows the sterile working conditions, preventing contamination frequently experienced in culture environment.
6) Gains knowledge of equipment in infertility or andrology laboratories that require cell culture knowledge.
7) Makes the definition of infertility and has an idea about the techniques used in laboratories.
8) Categorize buffer solutions, media and suspensions used in cell culture.
2 - Skills
Cognitive - Practical
1) Knows the cell based tests.
2) Learns cryopreservation methods in cell culture.
3 - Competences
Communication and Social Competence
Learning Competence
Field Specific Competence
Competence to Work Independently and Take Responsibility

Ders Akış Planı

Week	Subject	Related Preparation
1)	Introduction to Cell Culture and History	Basic Cell Culture Protocols, Cheryl D. HelgasonCindy L. Miller, 2005, Hücre Kültürüne Yeni Başlayanlar için Püf Noktaları : Dr. Öğr. Üyesi Gül İpek Gündoğan
2)	Cell Culture Laboratory Setup
3)	Materials and Equipment Used in Cell Culture
4)	Sterilization in Cell Culture
5)	Decontamination in Cell Culture
6)	Buffer Solutions, Media and Suspensions Used in Cell Culture
7)	Physical and Chemical Factors Affecting the Development of Cells in Culture
8)	Midterm exam
9)	Stem Cell and Application Areas-I
10)	Cell culture in infertility laboratories
11)	Midterm exam
12)	Viability Tests of Cells
13)	Cryopreservation in Cell Culture
14)	Cell-based tests
15)	Application Areas of Cell Culture
16)	Final Exam

Sources

Course Notes / Textbooks:	Basic Cell Culture Protocols, Cheryl D. HelgasonCindy L. Miller, 2005, Hücre Kültürüne Yeni Başlayanlar için Püf Noktaları : Dr. Öğr. Üyesi Gül İpek Gündoğan
References:	Basic Cell Culture Protocols, Cheryl D. HelgasonCindy L. Miller, 2005, Hücre Kültürüne Yeni Başlayanlar için Püf Noktaları : Dr. Öğr. Üyesi Gül İpek Gündoğan

Ders - Program Öğrenme Kazanım İlişkisi

Ders Öğrenme Kazanımları	1	2	3	4	5	6	7	10	8	9
Program Outcomes
1) Graduates have basic theoretical and practical knowledge about disinfection, sterilization and antisepsis.
2) Graduates have knowledge of ethical judgement .
3) Graduates use basic theoretical and applied knowledge about disinfection, sterilization and antisepsis.
4) Graduates carry out a task independently by using their basic knowledge of disinfection, sterilization and antisepsis.
5) Graduates determine their learning needs and have the competence to direct their own learning.
6) Graduates express themselves effectively by communicating both verbal and written by using information and communication technologies related to the field of disinfection, sterilization and antisepsis.
7) Graduates follow the developments in the agenda of the society and the world in the field of health and evaluate them with social responsibility awareness.
8) Graduates know the laws, regulations, legislation and professional ethical rules regarding their duties, rights and responsibilities and act in accordance with these rules.
9) Graduates can critically evaluate the basic knowledge and skills acquired in the field of disinfection, sterilization and antisepsis.
10) Graduates can inform the relevant people and institutions by using their basic knowledge and skills.
11) Graduates convey their thoughts on disinfection, sterilization and antisepsis at the level of basic knowledge and skills through written and verbal communication.
12) Graduates take responsibility as team members to solve complex unforeseen problems encountered in disinfection, sterilization and antisepsis applications.
13) Graduates act in accordance with quality management and processes, participate in these processes, have sufficient awareness of individual and public health, environmental protection and occupational safety.
14) With the awareness of social responsibility, it contributes to projects and activities for the social environment in which it lives, monitors the events on the agenda of the society and the world in the field of health and is sensitive to developments.

Ders - Öğrenme Kazanımı İlişkisi

No Effect	1 Lowest	2 Low	3 Average	4 High	5 Highest

	Program Outcomes	Level of Contribution
1)	Graduates have basic theoretical and practical knowledge about disinfection, sterilization and antisepsis.
2)	Graduates have knowledge of ethical judgement .
3)	Graduates use basic theoretical and applied knowledge about disinfection, sterilization and antisepsis.
4)	Graduates carry out a task independently by using their basic knowledge of disinfection, sterilization and antisepsis.
5)	Graduates determine their learning needs and have the competence to direct their own learning.
6)	Graduates express themselves effectively by communicating both verbal and written by using information and communication technologies related to the field of disinfection, sterilization and antisepsis.
7)	Graduates follow the developments in the agenda of the society and the world in the field of health and evaluate them with social responsibility awareness.
8)	Graduates know the laws, regulations, legislation and professional ethical rules regarding their duties, rights and responsibilities and act in accordance with these rules.
9)	Graduates can critically evaluate the basic knowledge and skills acquired in the field of disinfection, sterilization and antisepsis.
10)	Graduates can inform the relevant people and institutions by using their basic knowledge and skills.
11)	Graduates convey their thoughts on disinfection, sterilization and antisepsis at the level of basic knowledge and skills through written and verbal communication.
12)	Graduates take responsibility as team members to solve complex unforeseen problems encountered in disinfection, sterilization and antisepsis applications.
13)	Graduates act in accordance with quality management and processes, participate in these processes, have sufficient awareness of individual and public health, environmental protection and occupational safety.
14)	With the awareness of social responsibility, it contributes to projects and activities for the social environment in which it lives, monitors the events on the agenda of the society and the world in the field of health and is sensitive to developments.

Öğrenme Etkinliği ve Öğretme Yöntemleri

Anlatım
Course
Soru cevap/ Tartışma

Ölçme ve Değerlendirme Yöntemleri ve Kriterleri

Yazılı Sınav (Açık uçlu sorular, çoktan seçmeli, doğru yanlış, eşleştirme, boşluk doldurma, sıralama)

Assessment & Grading

Semester Requirements	Number of Activities	Level of Contribution
Midterms	1	% 40
Semester Final Exam	1	% 60
total		% 100
PERCENTAGE OF SEMESTER WORK		% 40
PERCENTAGE OF FINAL WORK		% 60
total		% 100

İş Yükü ve AKTS Kredisi Hesaplaması

Activities	Number of Activities	Duration (Hours)	Workload
Course Hours	14	2	28
Study Hours Out of Class	14	3	42
Midterms	2	4	8
Final	1	2	2
Total Workload			80