MOLECULAR BIOLOGY AND GENETICS (MASTER) (WHIT THESIS)
Master TR-NQF-HE: Level 7 QF-EHEA: Second Cycle EQF-LLL: Level 7

Ders Genel Tanıtım Bilgileri

Course Code: 3015002007
Ders İsmi: Functional Genomics
Ders Yarıyılı: Fall
Ders Kredileri:
Theoretical Practical Credit ECTS
3 0 3 6
Language of instruction:
Ders Koşulu:
Ders İş Deneyimini Gerektiriyor mu?: No
Type of course: Anabilim Dalı/Lisansüstü Seçmeli
Course Level:
Master TR-NQF-HE:7. Master`s Degree QF-EHEA:Second Cycle EQF-LLL:7. Master`s Degree
Mode of Delivery: Face to face
Course Coordinator : Prof. Dr. Meliha Burcu IRMAK YAZICIOĞLU
Course Lecturer(s):
Course Assistants:

Dersin Amaç ve İçeriği

Course Objectives: The course aims at giving the students an introduction to current topics in Functional Genomics research. The course also aims at presenting a variety of research techniques and their applications.
Course Content: Introduction to Functional Genomics and Basic Methods used in Functional Genomics Research, İsolation and Analysis of Total RNA and mRNA, Construction of Gene Libraries and their use Functional Genomics Research, Construction and Screening of Substractive Gene Libraries, Analysis of Changes in Gene Expression using Differential Display, Suppression Substractive Screenining Method, Serial Analysis of Gene Expression, Analysis of Gene Expression using Microarrays, Methods in Microarray Data Analysis, Protein Characterization Methods Used in Proteomics, Two Dimensional Gel Elecrophoresis, Protein Analysis using Mass Spektrometer, Bioinformatics : DNA and Protein Similarity Searches, Structural Modelling of Proteins

Learning Outcomes

The students who have succeeded in this course;
Learning Outcomes
1 - Knowledge
Theoretical - Conceptual
1) At the end of this course the students learn current functional genomic techniques available for gene and protein expression analysis.
2 - Skills
Cognitive - Practical
3 - Competences
Communication and Social Competence
Learning Competence
Field Specific Competence
1) Students learn how to evaluate data obtained in functional genomic studies.
2) At the end of this course the students can evaluate the applicability of these approaches to their own research.
Competence to Work Independently and Take Responsibility

Ders Akış Planı

Week Subject Related Preparation
1) Introduction to Functional Genomics and Basic Methods used in Functional Genomics Research Textbook and Lecture notes
2) İsolation and Analysis of Total RNA and mRNA Textbook and Lecture notes
3) Construction of Gene Libraries and their use Functional Genomics Research Textbook and Lecture notes
4) Construction and Screening of Substractive Gene Libraries Textbook and Lecture notes
5) Analysis of Changes in Gene Expression using Differential Display Textbook and Lecture notes
6) Suppression Substractive Screenining Method Textbook and Lecture notes
7) Serial Analysis of Gene expression Textbook and Lecture notes
8) Midterm
9) Analysis of Gene Expression using Microarrays Textbook and Lecture notes
10) Methods in Microarray Data Analysis Textbook and Lecture notes
11) Protein Characterization Methods Used in Proteomics Textbook and Lecture notes
12) Two Dimensional Gel Elecrophoresis Textbook and Lecture notes
13) Protein Analysis using Mass Spektrometer Textbook and Lecture notes
14) Bioinformatics : DNA and Protein Similarity Searches Textbook and Lecture notes
15) Final

Sources

Course Notes / Textbooks: Functional Genomics- Practical Approach Stephen P. Hunt and Frederick J. Livesey Oxford University Press 2000
Principles of Gene Manipulation Sandy B., Primrose, Richard M. Twyman, Madlen, MA; Oxford: Blackwell Pub., 2006
References: Functional Genomics- Practical Approach Stephen P. Hunt and Frederick J. Livesey Oxford University Press 2000
Principles of Gene Manipulation Sandy B., Primrose, Richard M. Twyman, Madlen, MA; Oxford: Blackwell Pub., 2006

Ders - Program Öğrenme Kazanım İlişkisi

Ders Öğrenme Kazanımları

1

2

3

Program Outcomes
1) Possess advanced level theoretical and practical knowledge supported by textbooks with updated information, practice equipments and other resources.
2) Use of advanced theoretical and practical knowledge within the field.
3) Interpret and evaluate data, define and analyze problems, develop solutions based on research and proofs by using acquired advanced knowledge and skills within the field.
4) Inform people and institutions, transfer ideas and solution proposals to problems in written and orally on issues in the field.
5) Share the ideas and solution proposals to problems on issues in the field with professionals and non-professionals by the support of qualitative and quantitative data.
6) Organize and implement project and activities for social environment with a sense of social responsibility.
7) Monitor the developments in the field and communicate with peers by using a foreign language at least at a level of European Language Portfolio B1 General Level.
8) Use informatics and communication technologies with at least a minimum level of European Computer Driving License Advanced Level software knowledge.
9) Evaluate the knowledge and skills acquired at an advanced level in the field with a critical approach.
10) Determine learning needs and direct the learning.
11) Develop positive attitude towards lifelong learning.
12) Act in accordance with social, scientific, cultural and ethic values on the stages of gathering, implementation and release of the results of data related to the field.
13) Possess sufficient consciousness about the issues of universality of social rights, social justice, quality, cultural values and also, environmental protection, worker's health and security.
14) Conduct studies at an advanced level in the field independently.
15) Take responsibility both as a team member and individually in order to solve unexpected complex problems faced within the implementations in the field.
16) Planning and managing activities towards the development of subordinates in the framework of a project.

Ders - Öğrenme Kazanımı İlişkisi

No Effect 1 Lowest 2 Low 3 Average 4 High 5 Highest
           
Program Outcomes Level of Contribution
1) Possess advanced level theoretical and practical knowledge supported by textbooks with updated information, practice equipments and other resources.
2) Use of advanced theoretical and practical knowledge within the field.
3) Interpret and evaluate data, define and analyze problems, develop solutions based on research and proofs by using acquired advanced knowledge and skills within the field.
4) Inform people and institutions, transfer ideas and solution proposals to problems in written and orally on issues in the field.
5) Share the ideas and solution proposals to problems on issues in the field with professionals and non-professionals by the support of qualitative and quantitative data.
6) Organize and implement project and activities for social environment with a sense of social responsibility.
7) Monitor the developments in the field and communicate with peers by using a foreign language at least at a level of European Language Portfolio B1 General Level.
8) Use informatics and communication technologies with at least a minimum level of European Computer Driving License Advanced Level software knowledge.
9) Evaluate the knowledge and skills acquired at an advanced level in the field with a critical approach.
10) Determine learning needs and direct the learning.
11) Develop positive attitude towards lifelong learning.
12) Act in accordance with social, scientific, cultural and ethic values on the stages of gathering, implementation and release of the results of data related to the field.
13) Possess sufficient consciousness about the issues of universality of social rights, social justice, quality, cultural values and also, environmental protection, worker's health and security.
14) Conduct studies at an advanced level in the field independently.
15) Take responsibility both as a team member and individually in order to solve unexpected complex problems faced within the implementations in the field.
16) Planning and managing activities towards the development of subordinates in the framework of a project.

Öğrenme Etkinliği ve Öğretme Yöntemleri

Alan Çalışması
Anlatım
Course
Homework
Rapor Yazma

Ölçme ve Değerlendirme Yöntemleri ve Kriterleri

Yazılı Sınav (Açık uçlu sorular, çoktan seçmeli, doğru yanlış, eşleştirme, boşluk doldurma, sıralama)
Homework
Sunum

Assessment & Grading

Semester Requirements Number of Activities Level of Contribution
Presentation 2 % 10
Midterms 1 % 30
Semester Final Exam 1 % 60
total % 100
PERCENTAGE OF SEMESTER WORK % 40
PERCENTAGE OF FINAL WORK % 60
total % 100

İş Yükü ve AKTS Kredisi Hesaplaması

Activities Number of Activities Duration (Hours) Workload
Course Hours 15 3 45
Study Hours Out of Class 15 5 75
Presentations / Seminar 5 3 15
Homework Assignments 10 4 40
Midterms 1 3 3
Final 1 3 3
Total Workload 181