MOLECULAR BIOLOGY AND GENETICS (MASTER) (WHIT THESIS)
Master TR-NQF-HE: Level 7 QF-EHEA: Second Cycle EQF-LLL: Level 7

Ders Genel Tanıtım Bilgileri

Course Code: 3015002006
Ders İsmi: Gene Therapy
Ders Yarıyılı: Fall
Ders Kredileri:
Theoretical Practical Credit ECTS
3 0 3 7
Language of instruction: TR
Ders Koşulu:
Ders İş Deneyimini Gerektiriyor mu?: No
Type of course: Anabilim Dalı/Lisansüstü Seçmeli
Course Level:
Master TR-NQF-HE:7. Master`s Degree QF-EHEA:Second Cycle EQF-LLL:7. Master`s Degree
Mode of Delivery: Face to face
Course Coordinator : Prof. Dr. Meliha Burcu IRMAK YAZICIOĞLU
Course Lecturer(s):
Course Assistants:

Dersin Amaç ve İçeriği

Course Objectives: Aim of this Course is to familiarize students with the most frequently used methods in Recombinant DNA Technology and Genetic Engineering from both a theoretical and experimental point of view.
Course Content: Recombinant DNA Technology and Genetic Engineering and Their Applications, DNA Restiction Enzymes, Cloning and Expression Vectors used for cloning, Gel Elecrophoresis of DNA and Proteins and Hybridization Techniques, Gene Transfer Techniques, DNA Sequence Analysis, Polymerase Chain Reaction, Construction and Use of Genomic and cDNA Libraries, Production of Recombinant Proteins, Production of Vaccines and Hormones in Microorganisms, Transgenic Plants and their Applications, Transgenic Animals and their applications, Impacts of Recombinant DNA and Genetic Engineering

Learning Outcomes

The students who have succeeded in this course;
Learning Outcomes
1 - Knowledge
Theoretical - Conceptual
1) Students learn basic methods of gene cloning and manipulation.
2 - Skills
Cognitive - Practical
3 - Competences
Communication and Social Competence
Learning Competence
Field Specific Competence
1) Students have an extended knowledge on vaccine and hormon production and transgenic plants.
2) Students can discuss the impacts of the recombinant DNA technology.
Competence to Work Independently and Take Responsibility

Ders Akış Planı

Week Subject Related Preparation
1) Recombinant DNA Technology and Genetic Engineering and Their Applications Textbook and Lecture notes
2) DNA Restiction Enzymes Textbook and Lecture notes
3) Cloning and Expression Vectors Textbook and Lecture notes
4) Gel Elecrophoresis of DNA and Proteins and Hybridization Techniques Textbook and Lecture notes
5) Gene Transfer Techniques Textbook and Lecture notes
6) DNA Sequence Analysis Textbook and Lecture notes
7) Polymerase Chain Reaction Textbook and Lecture notes
8) Midterm
9) Construction and Use of Genomic and cDNA Libraries Textbook and Lecture notes
10) Manipulating DNA in Microorganisms other than E. coli Textbook and Lecture notes
11) Recombinant Protein Production Textbook and Lecture notes
12) Production of Vaccines and Hormones in Microorganisms Textbook and Lecture notes
13) Transgenic Animals and their Applications Textbook and Lecture notes
14) Transgenic Animals and their Applications Textbook and Lecture notes
15) Final

Sources

Course Notes / Textbooks: Principles of Gene Manipulation Sandy B., Primrose, Richard M. Twyman, Madlen, MA; Oxford: Blackwell Pub., (2006)
Gene Cloning and DNA Analysis An Introduction Fourth Edition T.A Brown
References: Principles of Gene Manipulation Sandy B., Primrose, Richard M. Twyman, Madlen, MA; Oxford: Blackwell Pub., (2006)
Gene Cloning and DNA Analysis An Introduction Fourth Edition T.A Brown

Ders - Program Öğrenme Kazanım İlişkisi

Ders Öğrenme Kazanımları

1

2

3

Program Outcomes
1) Possess advanced level theoretical and practical knowledge supported by textbooks with updated information, practice equipments and other resources.
2) Use of advanced theoretical and practical knowledge within the field.
3) Interpret and evaluate data, define and analyze problems, develop solutions based on research and proofs by using acquired advanced knowledge and skills within the field.
4) Inform people and institutions, transfer ideas and solution proposals to problems in written and orally on issues in the field.
5) Share the ideas and solution proposals to problems on issues in the field with professionals and non-professionals by the support of qualitative and quantitative data.
6) Organize and implement project and activities for social environment with a sense of social responsibility.
7) Monitor the developments in the field and communicate with peers by using a foreign language at least at a level of European Language Portfolio B1 General Level.
8) Use informatics and communication technologies with at least a minimum level of European Computer Driving License Advanced Level software knowledge.
9) Evaluate the knowledge and skills acquired at an advanced level in the field with a critical approach.
10) Determine learning needs and direct the learning.
11) Develop positive attitude towards lifelong learning.
12) Act in accordance with social, scientific, cultural and ethic values on the stages of gathering, implementation and release of the results of data related to the field.
13) Possess sufficient consciousness about the issues of universality of social rights, social justice, quality, cultural values and also, environmental protection, worker's health and security.
14) Conduct studies at an advanced level in the field independently.
15) Take responsibility both as a team member and individually in order to solve unexpected complex problems faced within the implementations in the field.
16) Planning and managing activities towards the development of subordinates in the framework of a project.

Ders - Öğrenme Kazanımı İlişkisi

No Effect 1 Lowest 2 Low 3 Average 4 High 5 Highest
           
Program Outcomes Level of Contribution
1) Possess advanced level theoretical and practical knowledge supported by textbooks with updated information, practice equipments and other resources.
2) Use of advanced theoretical and practical knowledge within the field.
3) Interpret and evaluate data, define and analyze problems, develop solutions based on research and proofs by using acquired advanced knowledge and skills within the field.
4) Inform people and institutions, transfer ideas and solution proposals to problems in written and orally on issues in the field.
5) Share the ideas and solution proposals to problems on issues in the field with professionals and non-professionals by the support of qualitative and quantitative data.
6) Organize and implement project and activities for social environment with a sense of social responsibility.
7) Monitor the developments in the field and communicate with peers by using a foreign language at least at a level of European Language Portfolio B1 General Level.
8) Use informatics and communication technologies with at least a minimum level of European Computer Driving License Advanced Level software knowledge.
9) Evaluate the knowledge and skills acquired at an advanced level in the field with a critical approach.
10) Determine learning needs and direct the learning.
11) Develop positive attitude towards lifelong learning.
12) Act in accordance with social, scientific, cultural and ethic values on the stages of gathering, implementation and release of the results of data related to the field.
13) Possess sufficient consciousness about the issues of universality of social rights, social justice, quality, cultural values and also, environmental protection, worker's health and security.
14) Conduct studies at an advanced level in the field independently.
15) Take responsibility both as a team member and individually in order to solve unexpected complex problems faced within the implementations in the field.
16) Planning and managing activities towards the development of subordinates in the framework of a project.

Öğrenme Etkinliği ve Öğretme Yöntemleri

Alan Çalışması
Anlatım
Course
Homework

Ölçme ve Değerlendirme Yöntemleri ve Kriterleri

Yazılı Sınav (Açık uçlu sorular, çoktan seçmeli, doğru yanlış, eşleştirme, boşluk doldurma, sıralama)
Sözlü sınav
Homework
Sunum

Assessment & Grading

Semester Requirements Number of Activities Level of Contribution
Homework Assignments 7 % 10
Midterms 1 % 30
Semester Final Exam 1 % 60
total % 100
PERCENTAGE OF SEMESTER WORK % 40
PERCENTAGE OF FINAL WORK % 60
total % 100

İş Yükü ve AKTS Kredisi Hesaplaması

Activities Number of Activities Duration (Hours) Workload
Course Hours 15 3 45
Study Hours Out of Class 15 5 75
Homework Assignments 10 4 40
Midterms 1 3 3
Final 1 3 3
Total Workload 166