MOLECULAR BIOLOGY AND GENETICS (MASTER) (WHIT THESIS)
Master	TR-NQF-HE: Level 7	QF-EHEA: Second Cycle	EQF-LLL: Level 7

Ders Genel Tanıtım Bilgileri

Course Code:

3015002003

Ders İsmi:

Modern Techniques in Molecular Biology

Ders Yarıyılı:

Fall

Ders Kredileri:

Theoretical	Practical	Credit	ECTS
1	4	3	7

Language of instruction:

Ders Koşulu:

Ders İş Deneyimini Gerektiriyor mu?:

Type of course:

Anabilim Dalı/Lisansüstü Seçmeli

Course Level:

Master

TR-NQF-HE:7. Master`s Degree

QF-EHEA:Second Cycle

EQF-LLL:7. Master`s Degree

Mode of Delivery:

Face to face

Course Coordinator :

Prof. Dr. Meliha Burcu IRMAK YAZICIOĞLU

Course Lecturer(s):

Course Assistants:

Dersin Amaç ve İçeriği

Course Objectives:	This course is planned for graduate students to learn advanced molecular biological techniques are used in scientific research theoretically and practically, increase manual skills by applying in the laboratory.
Course Content:	Broad cross section of methodological approaches including cloning, genomic strategies, single molecule and single cell-based studies, in situ localization, high troughput approaches such as RNAi, gene expression analysis, genetic approaches with model systems as well as techniques for protein analysis.

Learning Outcomes

The students who have succeeded in this course;

Learning Outcomes

1 - Knowledge
Theoretical - Conceptual
1) Students learn general methods used in molecular biology laboratory.
2 - Skills
Cognitive - Practical
3 - Competences
Communication and Social Competence
Learning Competence
Field Specific Competence
1) Students learn how to apply molecular biology methods to their own research.
Competence to Work Independently and Take Responsibility

Ders Akış Planı

Week	Subject	Related Preparation
1)	Theory Topics: Methods Used in the Isolation and Purification of Macromolecules Practice Topics: DNA and RNA Isolation from Biological Materials
2)	Theory Topics: Types and Applications of PCR Practice Topics: Applications of PCR
3)	Theory Topics: Transfection Methods Practice Topics: Applications of Transfection
4)	Theory Topics: Protein Isolation Techniques Practice Topics: Isolation and Quantitation of Proteins from Biological Materials
5)	Theory Topics: Spectral Techniques-I Practice Topics: Spectrometry Applications
6)	Theory Topics: Spectral Techniques-II Practice Topics: ELISA Applications
7)	Theory Topics: Basic Flow Cytometric Methods Practice Topics: Basic Flow Cytometric Applications
8)	Midterm
9)	Theory Topics: Electrophoretic Techniques Practice Topics: Agarose Gel Electrophoresis of Nucleic Acids
10)	Theory Topics: Electrophoretic Techniques Practice Topics: Polyacrylamide Gel Eelectrophoresis of Protein
11)	Theory Topics: Technics Based on Blotting Practice Topics: Western Blotting
12)	Theory Topics: Advanced Microscopic Techniques-I Practice Topics: Fluorescence Labeling and Analysis-I
13)	Theory Topics: Advanced Microscopic Techniques-II Practice Topics: Fluorescence Labeling and Analysis-II
14)	Theory Topics: Advanced Microscopic Techniques-III Practice Topics: Observation of Fluorescence Labeling under a Microscope After Staining and the Solution of the Problems
15)	Final Exam

Sources

Course Notes / Textbooks:

Locquin and Langeron (1983). Handbook of Microscopy. Butterwaths
Ausubel et al (2002). Short Protocols in Molecular Biology. Wiley
Brown (2000). Essential Molecular Biology VI. AP
Brown (2000). Essential Molecular Biology VII. AP
Brown (2006). Gene Cloning and DNA Analysis - An Introduction. Blackwell
Glick and Pasternak (2003). Molecular Biotechnology. ASM Press
Kracher. Molecular Biology - A Practical Approach.
Krenzer and Massey (2000). Recombinant DNA and Biotechnology. ASM
Micklos and Freyer (1990). DNA Science. CSHL
Primrose (2001). Molecular Biotechnology. Panima
Robertson et al (1997). Manipulation & Expression of Recombinant DNA. AP
Sambrook et al (2001). Molecular Cloning. CSHL
Twyman (1999). Advanced Molecular Biology. Viva
Watson et al (1992). Recombinant DNA. Freeman
Primrose and Twyman (2006). Principles of Gene Manipulation and Genomics. Blackwell

References:

Ders - Program Öğrenme Kazanım İlişkisi

Ders Öğrenme Kazanımları	1	2
Program Outcomes
1) Possess advanced level theoretical and practical knowledge supported by textbooks with updated information, practice equipments and other resources.
2) Use of advanced theoretical and practical knowledge within the field.
3) Interpret and evaluate data, define and analyze problems, develop solutions based on research and proofs by using acquired advanced knowledge and skills within the field.
4) Inform people and institutions, transfer ideas and solution proposals to problems in written and orally on issues in the field.
5) Share the ideas and solution proposals to problems on issues in the field with professionals and non-professionals by the support of qualitative and quantitative data.
6) Organize and implement project and activities for social environment with a sense of social responsibility.
7) Monitor the developments in the field and communicate with peers by using a foreign language at least at a level of European Language Portfolio B1 General Level.
8) Use informatics and communication technologies with at least a minimum level of European Computer Driving License Advanced Level software knowledge.
9) Evaluate the knowledge and skills acquired at an advanced level in the field with a critical approach.
10) Determine learning needs and direct the learning.
11) Develop positive attitude towards lifelong learning.
12) Act in accordance with social, scientific, cultural and ethic values on the stages of gathering, implementation and release of the results of data related to the field.
13) Possess sufficient consciousness about the issues of universality of social rights, social justice, quality, cultural values and also, environmental protection, worker's health and security.
14) Conduct studies at an advanced level in the field independently.
15) Take responsibility both as a team member and individually in order to solve unexpected complex problems faced within the implementations in the field.
16) Planning and managing activities towards the development of subordinates in the framework of a project.

Ders - Öğrenme Kazanımı İlişkisi

No Effect	1 Lowest	2 Low	3 Average	4 High	5 Highest

	Program Outcomes	Level of Contribution
1)	Possess advanced level theoretical and practical knowledge supported by textbooks with updated information, practice equipments and other resources.
2)	Use of advanced theoretical and practical knowledge within the field.
3)	Interpret and evaluate data, define and analyze problems, develop solutions based on research and proofs by using acquired advanced knowledge and skills within the field.
4)	Inform people and institutions, transfer ideas and solution proposals to problems in written and orally on issues in the field.
5)	Share the ideas and solution proposals to problems on issues in the field with professionals and non-professionals by the support of qualitative and quantitative data.
6)	Organize and implement project and activities for social environment with a sense of social responsibility.
7)	Monitor the developments in the field and communicate with peers by using a foreign language at least at a level of European Language Portfolio B1 General Level.
8)	Use informatics and communication technologies with at least a minimum level of European Computer Driving License Advanced Level software knowledge.
9)	Evaluate the knowledge and skills acquired at an advanced level in the field with a critical approach.
10)	Determine learning needs and direct the learning.
11)	Develop positive attitude towards lifelong learning.
12)	Act in accordance with social, scientific, cultural and ethic values on the stages of gathering, implementation and release of the results of data related to the field.
13)	Possess sufficient consciousness about the issues of universality of social rights, social justice, quality, cultural values and also, environmental protection, worker's health and security.
14)	Conduct studies at an advanced level in the field independently.
15)	Take responsibility both as a team member and individually in order to solve unexpected complex problems faced within the implementations in the field.
16)	Planning and managing activities towards the development of subordinates in the framework of a project.

Öğrenme Etkinliği ve Öğretme Yöntemleri

Anlatım
Course
Okuma
Homework

Ölçme ve Değerlendirme Yöntemleri ve Kriterleri

Yazılı Sınav (Açık uçlu sorular, çoktan seçmeli, doğru yanlış, eşleştirme, boşluk doldurma, sıralama)
Sözlü sınav
Homework
Sunum

Assessment & Grading

Semester Requirements	Number of Activities	Level of Contribution
Homework Assignments	7	% 10
Midterms	1	% 30
Semester Final Exam	1	% 60
total		% 100
PERCENTAGE OF SEMESTER WORK		% 40
PERCENTAGE OF FINAL WORK		% 60
total		% 100

İş Yükü ve AKTS Kredisi Hesaplaması

Activities	Number of Activities	Duration (Hours)	Workload
Course Hours	15	3	45
Study Hours Out of Class	15	5	75
Presentations / Seminar	5	3	15
Homework Assignments	10	4	40
Midterms	1	3	3
Final	1	3	3
Total Workload			181