MOLECULAR BIOLOGY AND GENETICS (MASTER) (WHIT THESIS) | |||||
Master | TR-NQF-HE: Level 7 | QF-EHEA: Second Cycle | EQF-LLL: Level 7 |
Course Code: | 3015002003 | ||||||||
Ders İsmi: | Modern Techniques in Molecular Biology | ||||||||
Ders Yarıyılı: | Fall | ||||||||
Ders Kredileri: |
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Language of instruction: | TR | ||||||||
Ders Koşulu: | |||||||||
Ders İş Deneyimini Gerektiriyor mu?: | No | ||||||||
Type of course: | Anabilim Dalı/Lisansüstü Seçmeli | ||||||||
Course Level: |
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Mode of Delivery: | Face to face | ||||||||
Course Coordinator : | Prof. Dr. Meliha Burcu IRMAK YAZICIOĞLU | ||||||||
Course Lecturer(s): | |||||||||
Course Assistants: |
Course Objectives: | This course is planned for graduate students to learn advanced molecular biological techniques are used in scientific research theoretically and practically, increase manual skills by applying in the laboratory. |
Course Content: | Broad cross section of methodological approaches including cloning, genomic strategies, single molecule and single cell-based studies, in situ localization, high troughput approaches such as RNAi, gene expression analysis, genetic approaches with model systems as well as techniques for protein analysis. |
The students who have succeeded in this course;
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Week | Subject | Related Preparation |
1) | Theory Topics: Methods Used in the Isolation and Purification of Macromolecules Practice Topics: DNA and RNA Isolation from Biological Materials | |
2) | Theory Topics: Types and Applications of PCR Practice Topics: Applications of PCR | |
3) | Theory Topics: Transfection Methods Practice Topics: Applications of Transfection | |
4) | Theory Topics: Protein Isolation Techniques Practice Topics: Isolation and Quantitation of Proteins from Biological Materials | |
5) | Theory Topics: Spectral Techniques-I Practice Topics: Spectrometry Applications | |
6) | Theory Topics: Spectral Techniques-II Practice Topics: ELISA Applications | |
7) | Theory Topics: Basic Flow Cytometric Methods Practice Topics: Basic Flow Cytometric Applications | |
8) | Midterm | |
9) | Theory Topics: Electrophoretic Techniques Practice Topics: Agarose Gel Electrophoresis of Nucleic Acids | |
10) | Theory Topics: Electrophoretic Techniques Practice Topics: Polyacrylamide Gel Eelectrophoresis of Protein | |
11) | Theory Topics: Technics Based on Blotting Practice Topics: Western Blotting | |
12) | Theory Topics: Advanced Microscopic Techniques-I Practice Topics: Fluorescence Labeling and Analysis-I | |
13) | Theory Topics: Advanced Microscopic Techniques-II Practice Topics: Fluorescence Labeling and Analysis-II | |
14) | Theory Topics: Advanced Microscopic Techniques-III Practice Topics: Observation of Fluorescence Labeling under a Microscope After Staining and the Solution of the Problems | |
15) | Final Exam |
Course Notes / Textbooks: | Locquin and Langeron (1983). Handbook of Microscopy. Butterwaths Ausubel et al (2002). Short Protocols in Molecular Biology. Wiley Brown (2000). Essential Molecular Biology VI. AP Brown (2000). Essential Molecular Biology VII. AP Brown (2006). Gene Cloning and DNA Analysis - An Introduction. Blackwell Glick and Pasternak (2003). Molecular Biotechnology. ASM Press Kracher. Molecular Biology - A Practical Approach. Krenzer and Massey (2000). Recombinant DNA and Biotechnology. ASM Micklos and Freyer (1990). DNA Science. CSHL Primrose (2001). Molecular Biotechnology. Panima Robertson et al (1997). Manipulation & Expression of Recombinant DNA. AP Sambrook et al (2001). Molecular Cloning. CSHL Twyman (1999). Advanced Molecular Biology. Viva Watson et al (1992). Recombinant DNA. Freeman Primrose and Twyman (2006). Principles of Gene Manipulation and Genomics. Blackwell |
References: | Locquin and Langeron (1983). Handbook of Microscopy. Butterwaths Ausubel et al (2002). Short Protocols in Molecular Biology. Wiley Brown (2000). Essential Molecular Biology VI. AP Brown (2000). Essential Molecular Biology VII. AP Brown (2006). Gene Cloning and DNA Analysis - An Introduction. Blackwell Glick and Pasternak (2003). Molecular Biotechnology. ASM Press Kracher. Molecular Biology - A Practical Approach. Krenzer and Massey (2000). Recombinant DNA and Biotechnology. ASM Micklos and Freyer (1990). DNA Science. CSHL Primrose (2001). Molecular Biotechnology. Panima Robertson et al (1997). Manipulation & Expression of Recombinant DNA. AP Sambrook et al (2001). Molecular Cloning. CSHL Twyman (1999). Advanced Molecular Biology. Viva Watson et al (1992). Recombinant DNA. Freeman Primrose and Twyman (2006). Principles of Gene Manipulation and Genomics. Blackwell |
Ders Öğrenme Kazanımları | 1 |
2 |
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Program Outcomes | |||||||||||||||
1) Possess advanced level theoretical and practical knowledge supported by textbooks with updated information, practice equipments and other resources. | |||||||||||||||
2) Use of advanced theoretical and practical knowledge within the field. | |||||||||||||||
3) Interpret and evaluate data, define and analyze problems, develop solutions based on research and proofs by using acquired advanced knowledge and skills within the field. | |||||||||||||||
4) Inform people and institutions, transfer ideas and solution proposals to problems in written and orally on issues in the field. | |||||||||||||||
5) Share the ideas and solution proposals to problems on issues in the field with professionals and non-professionals by the support of qualitative and quantitative data. | |||||||||||||||
6) Organize and implement project and activities for social environment with a sense of social responsibility. | |||||||||||||||
7) Monitor the developments in the field and communicate with peers by using a foreign language at least at a level of European Language Portfolio B1 General Level. | |||||||||||||||
8) Use informatics and communication technologies with at least a minimum level of European Computer Driving License Advanced Level software knowledge. | |||||||||||||||
9) Evaluate the knowledge and skills acquired at an advanced level in the field with a critical approach. | |||||||||||||||
10) Determine learning needs and direct the learning. | |||||||||||||||
11) Develop positive attitude towards lifelong learning. | |||||||||||||||
12) Act in accordance with social, scientific, cultural and ethic values on the stages of gathering, implementation and release of the results of data related to the field. | |||||||||||||||
13) Possess sufficient consciousness about the issues of universality of social rights, social justice, quality, cultural values and also, environmental protection, worker's health and security. | |||||||||||||||
14) Conduct studies at an advanced level in the field independently. | |||||||||||||||
15) Take responsibility both as a team member and individually in order to solve unexpected complex problems faced within the implementations in the field. | |||||||||||||||
16) Planning and managing activities towards the development of subordinates in the framework of a project. |
No Effect | 1 Lowest | 2 Low | 3 Average | 4 High | 5 Highest |
Program Outcomes | Level of Contribution | |
1) | Possess advanced level theoretical and practical knowledge supported by textbooks with updated information, practice equipments and other resources. | |
2) | Use of advanced theoretical and practical knowledge within the field. | |
3) | Interpret and evaluate data, define and analyze problems, develop solutions based on research and proofs by using acquired advanced knowledge and skills within the field. | |
4) | Inform people and institutions, transfer ideas and solution proposals to problems in written and orally on issues in the field. | |
5) | Share the ideas and solution proposals to problems on issues in the field with professionals and non-professionals by the support of qualitative and quantitative data. | |
6) | Organize and implement project and activities for social environment with a sense of social responsibility. | |
7) | Monitor the developments in the field and communicate with peers by using a foreign language at least at a level of European Language Portfolio B1 General Level. | |
8) | Use informatics and communication technologies with at least a minimum level of European Computer Driving License Advanced Level software knowledge. | |
9) | Evaluate the knowledge and skills acquired at an advanced level in the field with a critical approach. | |
10) | Determine learning needs and direct the learning. | |
11) | Develop positive attitude towards lifelong learning. | |
12) | Act in accordance with social, scientific, cultural and ethic values on the stages of gathering, implementation and release of the results of data related to the field. | |
13) | Possess sufficient consciousness about the issues of universality of social rights, social justice, quality, cultural values and also, environmental protection, worker's health and security. | |
14) | Conduct studies at an advanced level in the field independently. | |
15) | Take responsibility both as a team member and individually in order to solve unexpected complex problems faced within the implementations in the field. | |
16) | Planning and managing activities towards the development of subordinates in the framework of a project. |
Anlatım | |
Course | |
Okuma | |
Homework |
Yazılı Sınav (Açık uçlu sorular, çoktan seçmeli, doğru yanlış, eşleştirme, boşluk doldurma, sıralama) | |
Sözlü sınav | |
Homework | |
Sunum |
Semester Requirements | Number of Activities | Level of Contribution |
Homework Assignments | 7 | % 10 |
Midterms | 1 | % 30 |
Semester Final Exam | 1 | % 60 |
total | % 100 | |
PERCENTAGE OF SEMESTER WORK | % 40 | |
PERCENTAGE OF FINAL WORK | % 60 | |
total | % 100 |
Activities | Number of Activities | Duration (Hours) | Workload |
Course Hours | 15 | 3 | 45 |
Study Hours Out of Class | 15 | 5 | 75 |
Presentations / Seminar | 5 | 3 | 15 |
Homework Assignments | 10 | 4 | 40 |
Midterms | 1 | 3 | 3 |
Final | 1 | 3 | 3 |
Total Workload | 181 |