| Course Objectives: |
Aim of this Course is to familiarize students with the most frequently used methods in Recombinant DNA Technology and Genetic Engineering from both a
theoretical and experimental point of view |
| Course Content: |
Genel Bilgiler, Rekombinant DNA ve Gen Mühendisliğinin Tanımı, Genel uygulama alanları, I.Rekombinant DNA Teknolojisinde Kullanılan Moleküler BiyolojikYöntemler, Genlerin Klonlanması ve Ekspresyonu, DNA Kesim Enzimleri, Gen aktarımında kullanılan klonlama ve ekspresyon vektörleri, DNA jel Elektroforezi,Hibridizasyon Yöntemleri, Gen aktarım Yöntemleri, DNA dizin analizi, Polimeraz Zincir Reaksiyonu, Genomik ve cDNA Kütüphaneleri ve kullanım alanları, GenEkspresyonu ve protein analizleri, II.Gen Mühendisliği Uygulamaları, Mikroorganizmalarda Rekombinant Protein Üretimi, Aşı ve Hormon Üretimi, Transgenik Bitkiler veKullanım Alanları, Transgenik Hayvanlar ve kullanım alanları, Rekombinant DNA Teknolojisi ve Genetik Mühendisliğinin Etkileri |
| Week |
Subject |
Related Preparation |
| 1) |
Recombinant DNA Technology and Genetic Engineering and Their Applications |
Gene Cloning and DNA Analysis An Introduction Fourth Edition T.A Brown
An Introduction to Genetic Engineering (Studies in Biology) (2002) Desmond S. T. Nicholl Cambridge University Press |
| 2) |
DNA Restiction Enzymes |
Gene Cloning and DNA Analysis An Introduction Fourth Edition T.A Brown
An Introduction to Genetic Engineering (Studies in Biology) (2002) Desmond S. T. Nicholl Cambridge University Press |
| 3) |
Cloning and Expression Vectors |
Gene Cloning and DNA Analysis An Introduction Fourth Edition T.A Brown
An Introduction to Genetic Engineering (Studies in Biology) (2002) Desmond S. T. Nicholl Cambridge University Press |
| 4) |
Gel Elecrophoresis of DNA and Proteins and Hybridization Techniques |
Principles of Gene Manipulation Sandy B., Primrose, Richard M. Twyman, Madlen, MA; Oxford: Blackwell Pub., (2006)
Gene Cloning and DNA Analysis An Introduction Fourth Edition T.A Brown |
| 5) |
Gene Transfer Techniques |
Principles of Gene Manipulation Sandy B., Primrose, Richard M. Twyman, Madlen, MA; Oxford: Blackwell Pub., (2006)
Gene Cloning and DNA Analysis An Introduction Fourth Edition T.A Brown |
| 6) |
DNA Sequence Analysis |
Principles of Gene Manipulation Sandy B., Primrose, Richard M. Twyman, Madlen, MA; Oxford: Blackwell Pub., (2006)
Gene Cloning and DNA Analysis An Introduction Fourth Edition T.A Brown |
| 7) |
Polymerase Chain Reaction |
Principles of Gene Manipulation Sandy B., Primrose, Richard M. Twyman, Madlen, MA; Oxford: Blackwell Pub., (2006)
Gene Cloning and DNA Analysis An Introduction Fourth Edition T.A Brown |
| 8) |
Midterm |
|
| 9) |
Construction and Use of Genomic and cDNA Libraries |
Principles of Gene Manipulation Sandy B., Primrose, Richard M. Twyman, Madlen, MA; Oxford: Blackwell Pub., (2006)
Gene Cloning and DNA Analysis An Introduction Fourth Edition T.A Brown |
| 10) |
Manipulating DNA in Microorganisms other than E. coli |
Principles of Gene Manipulation Sandy B., Primrose, Richard M. Twyman, Madlen, MA; Oxford: Blackwell Pub., (2006)
Gene Cloning and DNA Analysis An Introduction Fourth Edition T.A Brown |
| 11) |
Production of Recombinant Proteins |
Principles of Gene Manipulation Sandy B., Primrose, Richard M. Twyman, Madlen, MA; Oxford: Blackwell Pub., (2006)
Gene Cloning and DNA Analysis An Introduction Fourth Edition T.A Brown |
| 12) |
Production of Vaccines and Hormones in Microorganisms |
Principles of Gene Manipulation Sandy B., Primrose, Richard M. Twyman, Madlen, MA; Oxford: Blackwell Pub., (2006)
Gene Cloning and DNA Analysis An Introduction Fourth Edition T.A Brown |
| 13) |
Use of TransgenicPlants |
Principles of Gene Manipulation Sandy B., Primrose, Richard M. Twyman, Madlen, MA; Oxford: Blackwell Pub., (2006)
Gene Cloning and DNA Analysis An Introduction Fourth Edition T.A Brown |
| 14) |
Transgenic Animals and their Applications |
Principles of Gene Manipulation Sandy B., Primrose, Richard M. Twyman, Madlen, MA; Oxford: Blackwell Pub., (2006)
Gene Cloning and DNA Analysis An Introduction Fourth Edition T.A Brown |
| 15) |
Semester subject review |
|
| 16) |
Final exam |
|
| |
Program Outcomes |
Level of Contribution |
| 1) |
PÇ 1.1) To have sufficient knowledge required by the field of molecular biology and genetics |
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| 2) |
PO 1.2) To be able to use the theoretical knowledge required by the field of molecular biology and genetics |
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| 3) |
PO 1.3) To be able to apply the skills required by the field of molecular biology and genetics in the field |
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| 4) |
PO 2.1) To be able to identify, define and interpret the problems in the field of Molecular Biology and Genetics |
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| 5) |
PO 2.2) To be able to select appropriate analysis and modeling methods to solve problems in the field of Molecular Biology and Genetics |
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| 6) |
PO 2.3) To be able to apply appropriate analysis and modeling methods to solve problems in the field of Molecular Biology and Genetics |
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| 7) |
PO 3.1) To be able to understand and interpret any process, event, phenomenon, equipment or product |
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| 8) |
PO 3.2) To be able to solve the problems encountered with appropriate and contemporary methods |
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| 9) |
PO 4.) To be able to show interest in different disciplines outside the field for personal and professional development |
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| 10) |
PO 5.1) To be able to select and use modern tools necessary for Molecular Biology and Genetics applications |
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| 11) |
PO 5.2)To be able to use information technologies effectively for Molecular Biology and Genetics applications |
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| 12) |
PO 6) To be able to design, experiment, field study, data collection, data analysis, reporting, archiving, text solving and/or interpretation in the field of Molecular Biology and Genetics |
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| 13) |
PO 7) To be able to take responsibility as an individual and a team member, to produce solutions and to communicate well in unexpected complex situations that may be encountered in applications in the field of Molecular Biology and Genetics. |
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| 14) |
PO 8.1) To be able to communicate effectively both orally and in writing in Turkish in order to plan academic studies in the field of Molecular Biology and Genetics and to carry out them independently or jointly with stakeholders |
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| 15) |
PO 8.2) To be able to communicate orally and in writing in at least one foreign language in order to plan academic studies in the field of Molecular Biology and Genetics and to conduct them independently or jointly with stakeholders |
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| 16) |
PO 9) To have the awareness of the necessity of lifelong learning, to have the ability to access information, to follow developments in science and technology and to have the ability to constantly renew oneself. |
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| 17) |
PO 10) To have social, scientific, ethical values and awareness of protecting these values in the stages of collecting, interpreting, announcing and applying data related to the field of Molecular Biology and Genetics. |
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| 18) |
PO 11) To be able to comprehend the universal and social effects of Molecular Biology and Genetics applications (environmental problems, economy, sustainability, etc.) and legal aspects |
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BİLGİ PAKETİ / DERS KATALOĞU
