Course Objectives: |
The aim of this course is to illustrate mechanisms by which prokaryotic and eukaryotic cells regulate gene expression
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Course Content: |
Introduction, methods for studying transcriptional control, basics of prokaryotic transcriptional control, structure of promotor and RNA polymerase, operons:Lac and Trp operons, activation and repression mechanisms in prokaryotes, termination of transcription and translation in prokaryotes, eukaryotik transcriptional control: the eukaryotic promoter and assembly of the basal preinitiation complex, modulation of the activity of transcriptional activator proteins (phosphorylation, de novo synthesis , localisation), posttranscriptional control: alternative splicing, regulation of RNA transport and stability, control in translation, the role of chromatin as a repressor of transcription, post genomic studies in control of gene expression,importance of control in tissue spesific, developmental, stress dependent gene expression and in diseases including cancer.
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Week |
Subject |
Related Preparation |
1) |
Introduction: The basic Mechanisms involved in regulation of gene expression in eukoarotes and prokaryotes
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Textbook and Lecture notes
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2) |
Methods for studying transcriptional control (EMSA, DNA footprinting , protein-protein interaction assays)
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Textbook and Lecture notes
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3) |
Basics of prokaryotic transcriptional control, Structure of promotor and RNA polymerase
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Textbook and Lecture notes
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4) |
Operons:Lac and Trp operons
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Textbook and Lecture notes
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5) |
Activation and repression mechanisms in prokaryotes
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Textbook and Lecture notes
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6) |
Termination of transcription and translation in prokaryotes
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Textbook and Lecture notes
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7) |
Eukaryotik transcriptional control: The eukaryotic promoter and assembly of the basal preinitiation complex
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Textbook and Lecture notes
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8) |
Midterm |
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9) |
Activation and repression mechanisms of transcription in eukaryotes
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Textbook and Lecture notes
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10) |
Modulation of the activity of transcriptional activator proteins (phosphorylation, de novo synthesis , localisation)
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Textbook and Lecture notes
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11) |
Regulation of RNA transport and stability
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Textbook and Lecture notes
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12) |
Regulation of RNA transport and stability
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Textbook and Lecture notes
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13) |
Control in translation
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Textbook and Lecture notes
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14) |
The role of chromatin as a repressor of transcription
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Textbook and Lecture notes
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15) |
Final
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Course Notes / Textbooks: |
LEVIN’S GENES X, Jones and Bartlett 2011, Jocelyn E. Krebs, Elliott S. Goldstein, Stephen T. Kilpatrick
Eukaryotic Transcription Factors Fifth Edition, David Latchman, Elsevier, 2008 |
References: |
LEVIN’S GENES X, Jones and Bartlett 2011, Jocelyn E. Krebs, Elliott S. Goldstein, Stephen T. Kilpatrick
Eukaryotic Transcription Factors Fifth Edition, David Latchman, Elsevier, 2008 |
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Program Outcomes |
Level of Contribution |
1) |
Possess advanced level theoretical and practical knowledge supported by textbooks with updated information, practice equipments and other resources. |
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2) |
Use of advanced theoretical and practical knowledge within the field. |
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3) |
Interpret and evaluate data, define and analyze problems, develop solutions based on research and proofs by using acquired advanced knowledge and skills within the field. |
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4) |
Inform people and institutions, transfer ideas and solution proposals to problems in written and orally on issues in the field. |
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5) |
Share the ideas and solution proposals to problems on issues in the field with professionals and non-professionals by the support of qualitative and quantitative data. |
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6) |
Organize and implement project and activities for social environment with a sense of social responsibility. |
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7) |
Monitor the developments in the field and communicate with peers by using a foreign language at least at a level of European Language Portfolio B1 General Level. |
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8) |
Use informatics and communication technologies with at least a minimum level of European Computer Driving License Advanced Level software knowledge. |
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9) |
Evaluate the knowledge and skills acquired at an advanced level in the field with a critical approach. |
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10) |
Determine learning needs and direct the learning. |
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11) |
Develop positive attitude towards lifelong learning. |
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12) |
Act in accordance with social, scientific, cultural and ethic values on the stages of gathering, implementation and release of the results of data related to the field. |
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13) |
Possess sufficient consciousness about the issues of universality of social rights, social justice, quality, cultural values and also, environmental protection, worker's health and security. |
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14) |
Conduct studies at an advanced level in the field independently. |
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15) |
Take responsibility both as a team member and individually in order to solve unexpected complex problems faced within the implementations in the field. |
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16) |
Planning and managing activities towards the development of subordinates in the framework of a project. |
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